Translational Therapy Shared Resource
The Translational Therapy Shared Resource (TTSR) provides state-of-the-art translational research support services to Masonic Cancer Center members to facilitate the monitoring of clinical trials and the development of novel cellular and immune-based therapies. Transitioning sophisticated laboratory assays and the manufacturing of biotherapeutic products to the clinic can be difficult and often represents a significant hurdle in moving laboratory studies to the clinic. TTSR works directly with members of the scientific programs at the preclinical, clinical, and laboratory levels, with the goal of developing clinical trials to test novel therapies.
TTSR has 2 major components: Molecular and Cellular Therapeutics (MCT) and the Translational Therapy Laboratory (TTL).
Molecular and Cellular Therapeutics is a cGMP-compliant production facility. For more information about MCT visit their website.
The Translational Therapy Laboratory provides sample processing, immune monitoring, and biorepository management; it is the central laboratory that receives and processes research blood samples from patients enrolled in clinical trials. For more information about TTL Facilities, Services and Rates see below.
The immune monitoring and biorepository components of TTL are physically located in the Mayo Memorial Building, adjacent to the Masonic Cancer Research Building (MCRB) and the University of Minnesota Medical Center, Fairview.
The sample processing area (A410 Mayo) and an adjacent space used for specialized assays (A405 Mayo) contain biological safety cabinets, incubators, microscopes, counters, refrigerators, centrifuges, and freezers. A490/492 Mayo across the hall houses 2 -80C freezers, 5 large-capacity liquid nitrogen (LN2) storage tanks and a clinical-grade controlled-rate freezer.
A recently acquired satellite freezer storage space in F130 Diehl Hall currently houses 3 large-capacity LN2 storage tanks and 3 -80C freezers and provides sufficient space to house additional equipment necessary to accommodate a planned increase in banked specimens.
All TTL freezers are connected to PSECC, the University of Minnesota 24-hour electronic monitoring system, and TTL staff are notified if any equipment goes into alarm outside of working hours.
TTL uses the flow cytometers in the Flow Cytometry Shared Resource facility in MCRB and works closely with them.
In conjunction with the Clinical Trials Office (CTO) and the Clinical Informatics Shared Services (CISS) shared resources, TTL has created a reliable infrastructure to facilitate the collection and testing of research samples, as well as the retrieval of existing data and stored samples.
TTL licenses a Microsoft Access–based LIMS database application, MSC-LIMS, which allows for sample tracking, work-flow management, data entry, and data reporting. In addition, TTL uses the freezer inventory database, Freezerworks to manage the biorepository. MSC-LIMS and Freezerworks are supported by CISS and are part of the PI Dashboard application that provides integrated views of clinical and research data.
The Translational Therapy Shared Resource is a fee-for-service resource that provides correlative research sample processing and storage, and immune monitoring assays that include:
Chromium-51 based cytotoxicity assays
Flow cytometry-based functional assays to measure degranulation and cytokine production
ELISpot to detect antigen recognition
Intracellular cytokine staining to detect antigen recognition
MHC-multimer staining to detect antigen specific cytotoxic T-cells
Meeting investigators' needs
Clinical Trials Research Samples
Research sample processing and specialized testing services are available. We can also process, freeze and ship frozen samples to central repositories. We work closely with the Principal Investigator, Project Manager and Clinical Research Coordinator to prepare sample collection schedules, orders and request slips. The MSC-LIMS database allows us to track all immunotherapy patient samples and cellular therapy products from clinical research trials. Assay results are entered in MSC-LIMS.
The Translational Therapy Shared Resource operates as a good laboratory practice (GLP) facility. Well-trained personnel perform assays using standardized, validated protocols. We continually develop new methodologies to assess the in vivo immune effects and responses of novel clinical therapies as they are implemented and tested in patients.
TTL works closely with CISS to provide Principal Investigators with research data results integrated with clinical data in dashboards for monitoring results in real-time or as datasets for statistical analysis of translational research trials.
To Use Our Services
To use our services, you will need to create a project in the CTSI portal and initiate a request for TTL services. We require a copy of a clinical protocol or laboratory manual, including a schedule of samples to be collected and services to be provided, so that we can prepare a budget estimate. If you choose to use TTL services, we will need confirmation of an IRB-approved protocol and a Chart String before we can accept samples in the lab. Billing is also done via the CTSI portal.
We encourage you to contact us during the protocol design for assistance in determining the optimal sample collection time and laboratory assays to answer your research questions. We can provide scientific expertise and procedural support to assist you with the institutional review process and the logistics of collecting research samples as part of a clinical trial.
Questions about assay prices, cost estimates for specialized products and services, and requests for any other information may be directed to:
Rose Wangen, MPH, Coordinator, at 612-625-6955 or by email at email@example.com
Martin Felices, Co-Director, at 612-625-8942 or by e-mail at firstname.lastname@example.org
FY20 rates will go into effect on 7/1/19. Websites will be updated with the FY20 rates by July 12, 2019. Please contact the shared resource manager or coordinator with questions.
Services and rates for fiscal year 2019 rates
Masonic Cancer Center members receive discounted rates.
Please contact the TTL for additional information.
|Lab hourly||Non-standard assays; rate includes $28 of supplies and reagents per hour billed; customer will be billed separately if supply costs exceed this allowance||$158.48|
|Cell Process||Cell Process 1 (no storage)||No ficoll, freeze MNC OR DNA only, up to 20 tubes; customer picks up cryovials within 1 week||$63.68|
|Cell Process 1||No ficoll, freeze MNC OR DNA only, up to 20 tubes; customer picks up cryovials within 1 week||$75.19|
|Cell Process 2 (no storage)||Ficoll, cell counts, freeze MNC up to 20 tubes; customer picks up cryovials within 1 week||$116.90|
|Cell Process 2||Ficoll, cell counts, freeze MNC up to 20 tubes||$128.42|
|Cell Process 3||Freeze lysed post-ficoll pellet for DNA, 4 tubes
|Cell Process - Cytoreduction||Ficoll, count, freeze up to 300 vials||$623.24|
|Freezer storage, 20 increments > 20||If > 20 tubes frozen, additional charge per 20 tubes||$33.41|
|Other Sample Process||Whole blood, aliquot and freeze||Prepare up to 4 aliquots of whole blood, store at -80C||$13.09|
|Serum, Plasma, or Urine, simple, no storage||Blood or Urine: standard spin and aliquot, no long term storage||$18.88|
|Serum, Plasma, or Urine, simple||Blood or Urine: standard spin, aliquot and store||$29.25|
|Serum, Plasma, or Urine, complex, no storage||Customer picks up cryovials within 1 week||$46.52|
|Serum, Plasma, or Urine, complex||Blood or Urine: non-standard or multiple spins, with aliquot and store||$58.04|
|Sample - no process, no storage||Blood or other||$20.73|
|Sample - no process||Blood or other: pick up frozen specimens from other processing lab, store||$31.09|
|Shipping - labor only||Cost per box; customer provides shipping supplies||$69.10|
|Shipping - labor, box, dry ice (domestic)||Cost per box; TTL provides shipping supplies||$100.53|
|Shipping - labor only (international)||Cost per box; customer provides shipping supplies||$103.64|
|Shipping - labor, box, dry ice (international)||Cost per box; labor, box, dry ice||$135.08|
|Cytoxicity||Cytotoxicity - 1 target||MNC: measure killing of one cell type (target) by Cr51 release||$216.56|
|Cytotoxicity - 2 targets||MNC: measure killing of two cell types (targets) by Cr51 release||$302.68|
|Phenotype||FACS, 10 color surface, 5-6 tubes||Cells: stain up to 10 surface markers in 5-6 tubes; acquire; analyze; report||$258.56|
|FACS, 10 color surface, 3-4 tubes||Cells: stain up to 10 surface markers in 3-4 tubes; acquire; analyze; report||$213.13|
|FACS, 10 color surface, 1-2 tubes||Cells: stain up to 10 surface markers in 1-2 tubes; acquire; analyze; report||$178.84|
|FACS, 10 color intracellular, 1-2 tubes||Cells: stain up to 10 surface and intracellular markers in 1-2 tubes; acquire; analyze; report||$194.92|
|FACS, 10 color intracellular, 3-4 tubes||Cells: stain up to 10 surface and intracellular markers in 3-4 tubes; acquire; analyze; report||$224.09|
|FACS, 10 color whole blood, 1-2 tubes||Blood: stain up to 10 surface markers in whole blood in 1-2 tubes; lyse RBCs; acquire; analyze; report||$203.58|
|Functional tests||CD107a/ Intracellular cytokine||KIR||$389.60|
|Column enrichment, NK cells or CD4 cells||Previous name: CD4 enrichment (MS column); now includes column enrichment, NK cells||$270.47|
|Neutralization Assay||New for FY18; this test was developed for HM2014-26||$217.92|
|CFSE Suppression Assay||Previous name: CFSE (does not include MS column)||$329.68|
|ELISpot, per sample||Previous name: ELISpot; study specific antigens or other reagents will be billed directly to study||$366.06|
|Pentamer- 6 stains||Per sample, up to 6 stains; pentamers will be billed directly to study||$218.49|
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