Translational Therapy Shared Resource

The Translational Therapy Shared Resource (TTSR) provides state-of-the-art translational research support services to Masonic Cancer Center members to facilitate the monitoring of clinical trials and the development of novel cellular and immune-based therapies. Transitioning sophisticated laboratory assays and the manufacturing of biotherapeutic products to the clinic can be difficult and often represents a significant hurdle in moving laboratory studies to the clinic. TTSR works directly with members of the scientific programs at the preclinical, clinical, and laboratory levels, with the goal of developing clinical trials to test novel therapies.

TTSR has 2 major components: Molecular and Cellular Therapeutics (MCT) and the Translational Therapy Laboratory (TTL).

  • Molecular and Cellular Therapeutics is a cGMP-compliant production facility. For more information about MCT visit their website.

  • The Translational Therapy Laboratory provides sample processing, immune monitoring, and biorepository management; it is the central laboratory that receives and processes research blood samples from patients enrolled in clinical trials. For more information about TTL Facilities, Services and Rates see below.

Facilities

Facilities

The immune monitoring and biorepository components of TTL are physically located in the Mayo Memorial Building, adjacent to the Masonic Cancer Research Building (MCRB) and the University of Minnesota Medical Center, Fairview.

The sample processing area (A410 Mayo) and an adjacent space used for specialized assays (A405 Mayo) contain biological safety cabinets, incubators, microscopes, counters, refrigerators, centrifuges, and freezers. A490/492 Mayo across the hall houses 2 -80C freezers, 5 large-capacity liquid nitrogen (LN2) storage tanks and a clinical-grade controlled-rate freezer.

A recently acquired satellite freezer storage space in F130 Diehl Hall currently houses 3 large-capacity LN2 storage tanks and 3 -80C freezers and provides sufficient space to house additional equipment necessary to accommodate a planned increase in banked specimens.

All TTL freezers are connected to PSECC, the University of Minnesota 24-hour electronic monitoring system, and TTL staff are notified if any equipment goes into alarm outside of working hours.

TTL uses the flow cytometers in the Flow Cytometry Shared Resource facility in MCRB and works closely with them.

In conjunction with the Clinical Trials Office (CTO) and the Clinical Informatics Shared Services (CISS) shared resources, TTL has created a reliable infrastructure to facilitate the collection and testing of research samples, as well as the retrieval of existing data and stored samples.

TTL licenses a Microsoft Access–based LIMS database application, MSC-LIMS, which allows for sample tracking, work-flow management, data entry, and data reporting. In addition, TTL uses the freezer inventory database, Freezerworks to manage the biorepository. MSC-LIMS and Freezerworks are supported by CISS and are part of the PI Dashboard application that provides integrated views of clinical and research data.

Services

Services

The Translational Therapy Shared Resource is a fee-for-service resource that provides correlative research sample processing and storage, and immune monitoring assays that include:

  • Immunophenotyping

  • Chromium-51 based cytotoxicity assays

  • Flow cytometry-based functional assays to measure degranulation and cytokine production

  • ELISpot to detect antigen recognition

  • Intracellular cytokine staining to detect antigen recognition

  • MHC-multimer staining to detect antigen specific cytotoxic T-cells

Meeting investigators' needs

Clinical Trials Research Samples

Research sample processing and specialized testing services are available. We can also process, freeze and ship frozen samples to central repositories. We work closely with the Principal Investigator, Project Manager and Clinical Research Coordinator to prepare sample collection schedules, orders and request slips. The MSC-LIMS database allows us to track all immunotherapy patient samples and cellular therapy products from clinical research trials. Assay results are entered in MSC-LIMS.

Quality

The Translational Therapy Shared Resource operates as a good laboratory practice (GLP) facility. Well-trained personnel perform assays using standardized, validated protocols. We continually develop new methodologies to assess the in vivo immune effects and responses of novel clinical therapies as they are implemented and tested in patients.

Research Results

TTL works closely with CISS to provide Principal Investigators with research data results integrated with clinical data in dashboards for monitoring results in real-time or as datasets for statistical analysis of translational research trials.

To Use Our Services

To use our services, you will need to create a project in the CTSI portal and initiate a request for TTL services. We require a copy of a clinical protocol or laboratory manual, including a schedule of samples to be collected and services to be provided, so that we can prepare a budget estimate. If you choose to use TTL services, we will need confirmation of an IRB-approved protocol and a Chart String before we can accept samples in the lab. Billing is also done via the CTSI portal.

We encourage you to contact us during the protocol design for assistance in determining the optimal sample collection time and laboratory assays to answer your research questions. We can provide scientific expertise and procedural support to assist you with the institutional review process and the logistics of collecting research samples as part of a clinical trial.

Contact

Questions about assay prices, cost estimates for specialized products and services, and requests for any other information may be directed to:

Rose Wangen, MPH,  Coordinator, at 612-625-6955 or by email at dewa0051@umn.edu

Martin Felices, Co-Director, at 612-625-8942 or by e-mail at mfelices@umn.edu

Rates

Rates

Services and rates for fiscal year 2020 rates

Masonic Cancer Center members receive discounted rates.
Please contact the TTL for additional information.

Category Test Description Rate
  Lab Hourly Non-standard assays; rate includes $28 of supplies and reagents per hour billed; customer will be billed separately if supply costs exceed this allowance $142.21
Cell Process
Cell Process 1 no ficoll, count, wash and freeze MNC, up to 10 cryovials, store in liquid nitrogen in TTL $73.66
Cell Process 1,
no storage
no ficoll, count, wash and freeze MNC, up to 10 cryovials; temporary storage in liquid nitrogen for up to 1 month $63.31
Cell Process 2 ficoll, cell count pre and post ficoll, freeze MNC, up to 10 cryovials, store in liquid nitrogen in TTL $121.99
Cell Process 2,
no storage
ficoll, cell count pre and post ficoll, freeze MNC, up to 10 cryovials; temporary storage in liquid nitrogen for up to 1 month $111.64
Cell Process 3 ficoll, cell count pre and post ficoll, freeze MNC, up to 10 cryovials, store in liquid nitrogen in TTL AND freeze lysed post-ficoll pellet (mainly granulocytes) for DNA, 4 cryovials, store at -80C in TTL $150.75
Cell Process- Cytoreduction count, wash and freeze MNC, up to 300 cryovials, store in liquid nitrogen in TTL $577.87
Other Sample Process
Aliquot and store aliquot sample without processing into up to 10 cryovials, store at -80C in TTL $22.42
Aliquot, no storage aliquot sample without processing into up to 10 cryovials; temporary storage at -80C for up to 1 month $17.24
Process (simple), aliquot and store Process sample by standard centrifugation or simple process, aliquot into up to 10 cryovials, store at -80C in TTL $27.60
Process (simple), aliquot, no storage Process sample by standard centrifugation or simple process, aliquot into up to 10 cryovials; temporary storage at -80C for up to 1 month $22.42
Process (complex), aliquot and store Process sample by non-standard or multiple centrifugation steps, or other complex process, aliquot into up to 10 cryovials, store at -80C in TTL $49.25
Process (complex), aliquot, no storage Process sample by non-standard or multiple centrifugation steps, or other complex process, aliquot into up to 10 cryovials; temporary storage at -80C for up to 1 month $44.08
Sample (single), login and store Receive sample (single sample type and time point), login, label and store in TTL $20.70
Sample (single), login, no storage Receive sample (single sample type time point), login and label; temporary storage for up to 1 month OR login and transfer to antother lab $15.53
Sample (batch), login and store Receive a batch of samples (multiple samples types and/or time points), login, label and store in TTL $31.05
Sample (batch), login, no storage Receive a batch of samples (multiple samples types and/or time points), login and label; temporary storage for up to 1 month $25.88
Freezer storage
Freezer storage, > 10, increments of 20 if >10 cryovials frozen, additional charge per 20 cryovials stored in liquid nitrogen or at -80C in TTL $30.24
Legacy freezer storage: LN2 2 years after the last sample from a study is received TTL will begin charging inidcated rate every 6 months for each box (100 cryovials) in LN2 storage, or the investigator can retrieve the samples. $51.76
Legacy freezer storage: -80C 2 years after the last sample from a study is received TTL will begin charging inidcated rate every 6 months for each box (81 cryovials) in -80C storage, or the investigator can retrieve the samples. $15.53
Shipping
Shipping - labor only
Domestic
per box; study provides box, dry ice, fedex $62.11
Shipping - labor, box, dry ice
Domestic
per box; study provides fedex $90.18
Shipping - labor only
International
per box; study provides box, dry ice, international courier $93.16
Shipping - labor, box, dry ice
International
per box; study provides international courier $121.24
Cytotoxicity
Cytotoxicity - 1 target MNC: measure killing of one cell type (target) by Cr51 release $194.35
Cytotoxicity - 2 targets MNC: measure killing of two cell types (targets) by Cr51 release $271.40
Phenotype
FACS, 10 color surface, 5-6 tubes includes staining, acquisition, analysis, database import $219.59
FACS, 10 color surface, 3-4 tubes includes staining, acquisition, analysis, database import $183.46
FACS, 10 color surface, 1-2 tubes includes staining, acquisition, analysis, database import $152.94
FACS, 10 color intracellular, 1-2 tubes includes staining, acquisition, analysis, database import $183.09
FACS, 10 color intracellular, 3-4 tubes includes staining, acquisition, analysis, database import $201.07
FACS, 10 color whole blood, 1-2 tubes includes staining, acquisition, analysis, database import $177.66
Functional Tests
CD107a/ Intracellular cytokine, 1 test condition includes culture, staining, acquisition, analysis, database import; includes 2 controls and 1 experimental condition $348.63
CD107a/ Intracellular cytokine, 2-3 test conditions includes culture, staining, acquisition, analysis, database import; includes 2 controls and 2-3 experimental conditions $449.35
Column enrichment, NK cells or CD4 cells previous name: CD4 enrichment (MS column); now includes column enrichment, NK cells $242.76
Neutralization Assay new for FY18; this test was developed for HM2014-26 $232.22
CFSE Suppression Assay previous name: CFSE (does not include MS column) $295.78
ELISpot, per sample previous name: ELISpot; study specific antigens or other reagents will be billed directly to study $328.54
Pentamer- 6 stains per sample, up to 6 stains; pentamers will be billed directly to study $195.94

 

 

 

Heme Malignancy Tissue Bank

Heme Malignancy Tissue Bank

Please visit DBUC website for HMTB sample application.

Staff

Co-directors
John Wagner, M.D.

Office: 660D Masonic Cancer Center Research Building
612-626-0474
wagne002@umn.edu

Martin Felices, Ph.D.
Office: 660A CCRB
612-625-8942
mfelices@umn.edu

Coordinator
Julie Curtsinger, Ph.D. 

612-625-6955

curts001@umn.edu

Video on services provided