Genome Engineering

Genome engineering bannerThe Genome Engineering Shared Resource (GESR) is a Core dedicated for the use of all Masonic Cancer Center members. Its purpose is to provide (at cost) state-of-the-art genome editing services for human cell lines. The GESR will initially have the capacity to make standard knockouts and knockins for your favorite gene in your favorite cell line. Ultimately GESR will provide services for making conditional alleles and epitope tagging endogenous genes as well as carrying out whole genome knockout screens.

The goal of the GESR is to utilize state-of-the-art CRISPR/Cas9-mediated gene targeting to efficiently generate desired modifications in mammalian cells in a timely manner in order to accelerate the individual researchers intellectual pursuit.

Watch a video on genome editing from the McGovern Institute.

Gene Editing 101 Seminar

Gene Editing 101

New service: Creating mutant mice by CRISPR/Cas9 approach

In light of the increasing mouse genome editing via CRISPR/Cas9 approach, the Genome Engineering Shared Resource (GESR) and Mouse Genetic Laboratory (MGL) have teamed up to provide seamless service for investigators at University of Minnesota, starting from design of CRISPR/cas9 reagents, generation and validation in cell line of the designed products, and microinjection of these reagents to produce mutant mice. Contact the GESR coordinator Brian Ruis, ruisx001@umn.edu, or MGL coordinator Yun Yu, yyou@umn.edu.
 

Services

Services

All gene modifications will be generated using CRISPR/Cas9 targeting vectors as well as modified versions of those vectors.

  1. Non-essential gene knockout
  2. Conditional knockout for essential genes
  3. Knock-in of desired mutations

Future services to be provided:

  • Whole genome wide CRISPR screens
  • Gene tagging

Flow cytometry services are provided by the University Flow Cytometry Resource (UFCR).

Rates

Rates

GESR Services and rates for fiscal year 2021 rates
Services and Fiscal Year 2021 Rates
     
MCC Member Rate Non-MCC Member Rate External Rate
Non-Phased Billing    
Consulting: Hourly rate of $204.00 billed at $51.00 every 15 minutes $106.32 every 15min/$425.28 Hourly Rate $173.3 every 15 min/693.20 Hourly Rate
sgRNA Design Service: $133.03 $258.03 $420.59
sgRNA Expression Vector Construction: $400.52 $470.52 $766.95
sgRNA Validation: $397.84 $497.84 $811.48
Cas9 Expression Vector Reagents: $66.68 $100.18 $163.29
HDR Donor Vector Construction: $1,000 - NEW SERVICE FY21 $1,051.00 $1,713.13
     
Phased Billing    
Simple, Single Locus KOs for Non-Essential Gene: $3,423.87 $6,923.87 $11,285.91
Construction of Conditionally-Null Alleles for Essential Gene: $7,917.51 $10,017.51 $16,328.54
Epitope Tagging: $6,601.28 $8,101.28 $13,205.09
Construction of Single Amino Acid Knock-In Mutation: $5,328.34 $6,678.34 $10,885.69
Construction of Single NT Knock-In SNPs: $5,197.98 $6,647.98 $10,836.21
Construction of Multi-Allelic KO Cell Lines: $4,618.34 $6,678.34 $10,885.69
Rudimentary Cas9/CRISPR Whole Genome (&/or selected pathway) Library Screens: $15,894.24 $16,644.24 $27,130.11
* Services that require "Phased Billing" will be billed incrementally upon completion of each phase of the given process. Please contact GESR staff if you have questions about the phased billing method and when you can expect to be charged for GESR services.

Service requests

Service requests

Genome Engineering request form

Each customer should provide as much information about the cell line they are targeting and the desired modification so that initial planning can be started before the first consultation meeting is set up.

Download the Service Request Form

Sample Submission

Cell lines to be engineered can be submitted either frozen or as a live culture in a sealed cell culture flask between the hours of 9 a.m. and 3 p.m.

OR

Arrange their pickup with our lab staff contact.  See staff list.

Turn-around time

Projects will be started in the order they are received.

The amount of time estimated to complete the genome engineering process will be determined on a case-by-case basis.

Pick-up of produced products

Completed cell lines can be picked up as either a frozen stock or as a live culture provided in a sealed T25 flask from 6-108 MCB.

Cytogenomics

Investigators are encouraged to submit their chosen cell line to the Cytogenomics Shared Resource for G-band Karyotyping to determine the ploidy status and genomic integrity before proceeding with genetic modification by the GESR. 

Location

Location

We are located at:

Laboratory

6-221 Molecular Cellular Biology Building
420 Washington Ave SE
Minneapolis, MN 55455

Office

6-108 Molecular Cellular Biology Building
420 Washington Ave SE
Minneapolis, MN 55455

mcbb map

iLab

iLab

Going forward, the MCC- Genome Engineering at the University of Minnesota is excited to start using an online system to streamline the process of requesting and billing for core services. All facility users are invited to use the system, which requires a one-time registration as discussed below. Once you are registered, the system will enable you to place service requests, provide required approvals, and monitor progress.

To register for an account:

To get started, you must register for an account:

  1. Navigate to the core page:

https://umn.corefacilities.org/service_center/show_external/4828

  1. In the upper-right-hand corner click ‘LOGIN’ and you should be directed to the UMN Sign In page.

  2. Login using your UMN credentials.

  3. Upon successful authentication, you will be prompted to select your PI/lab from the pull-down list and provide your phone number.

  4. Submit the completed registration form. Submission will notify your PI of your lab membership request.

  5. You will be sent an email with basic instructions on using the system at this time. However, you will not be able to place orders with your new account until your PI has approved your lab membership request.

To create a service request:

Once you have been accepted into your PI’s lab and assigned an EFS Account Strings, you can submit requests.

  1. Navigate to the core page:

https://umn.corefacilities.org/service_center/show_external/4828

  1. In the upper-right-hand corner click ‘LOGIN’ A pop-up window should appear displaying, ‘Sign in using UMN (with UMN ID) credentials’ Click the here link.

  2. Enter your user id and password, and sign in.

  3. Select the Request Services tab and click on the ‘Request Service’ button next to the service of interest.

  4. You will be asked to complete a form and provide payment information for your request before submitting the request to the core.

  5. Your request will be pending review by the core. The core will add charges and submit it back to you for approval. Make sure to watch for an email from iLab regarding your updated project. 

Additional help

More detailed instructions are available on our helpsite. For any questions not addressed, click on the “HELP” link in the upper right hand corner or contact ilab-support@agilent.com.

Sincerely,

The iLab - University of Minnesota Team

Staff

Co-directors

Eric Hendrickson, Ph.D
Office: 6-102 MCB
612-624-5988
hendr064@umn.edu

Branden Moriarity, Ph.D.
Office: 3-128 CCRB
612-625-2226
mori0164@umn.edu

Coordinator

Brian Ruis
Office: 6-108 MCB
612-624-0935
612-625-1484
ruisx001@umn.edu

Support staff

Huiyan (Summer) Ma
Office: 6-108 MCB
612-624-0935
maxx0326@umn.edu