Genome Engineering

Genome engineering bannerThe Genome Engineering Shared Resource (GESR) is a developing Core dedicated for the use of all Masonic Cancer Center members. Its purpose is to provide (at cost) state-of-the-art genome editing services for human cell lines. The GESR will initially have the capacity to make standard knockouts and knockins for your favorite gene in your favorite cell line. Ultimately GESR will provide services for making conditional alleles and epitope tagging endogenous genes as well as carrying out whole genome knockout screens.

The goal of the GESR is to utilize state-of-the-art CRISPR/Cas9-mediated gene targeting to efficiently generate desired modifications in mammalian cells in a timely manner in order to accelerate the individual researchers intellectual pursuit.

Watch a video on genome editing from the McGovern Institute.

Gene Editing 101 Seminar

Gene Editing 101

New service: Creating mutant mice by CRISPR/Cas9 approach

In light of the increasing mouse genome editing via CRISPR/Cas9 approach, the Genome Engineering Shared Resource (GESR) and Mouse Genetic Laboratory (MGL) have teamed up to provide seamless service for investigators at University of Minnesota, starting from design of CRISPR/cas9 reagents, generation and validation in cell line of the designed products, and microinjection of these reagents to produce mutant mice. Contact the GESR coordinator Brian Ruis, ruisx001@umn.edu, or MGL coordinator Yun Yu, yyou@umn.edu.
 

Services

Services

All gene modifications will be generated using CRISPR/Cas9 targeting vectors as well as modified versions of those vectors.

  1. Non-essential gene knockout
  2. Conditional knockout for essential genes
  3. Knock-in of desired mutations

Future services to be provided:

  • Whole genome wide CRISPR screens
  • Gene tagging

Flow cytometry services are provided by the University Flow Cytometry Resource (UFCR).

Rates

Rates

Services and Fiscal Year 2018 Rates

Non-Phased Billing

Consulting: Hourly rate of $182.72 billed at $45.68 every 15 minutes

sgRNA Design Service: $131.66

sgRNA Expression Vector Construction: $395.16

sgRNA Validation: $394.99

Cas9 Expression Vector Reagents: $65.83

Phased Billing

Simple, Single Locus KOs for Non-Essential Gene: $3,994.65

Construction of Conditionally-Null Alleles for Essential Gene: $7,820.71

Construction of Single Amino Acid Knock-In Mutation: $5,266.51

Construction of Single NT Knock-In SNPs: $5,158.57

Construction of Multi-Allelic KO Cell Lines: $4,564.11

Rudimentary Cas9/CRISPR Whole Genome (&/or selected pathway) Library Screens: $15,732.39

* Services that require "Phased Billing" will be billed incrementally upon completion of each phase of the given process. Please contact GESR staff if you have questions about the phased billing method and when you can expect to be charged for GESR services.

Service requests

Service requests

Genome Engineering request form

Each customer should provide as much information about the cell line they are targeting and the desired modification so that initial planning can be started before the first consultation meeting is set up.

Download the Service Request Form

Sample Submission

Cell lines to be engineered can be submitted either frozen or as a live culture in a sealed cell culture flask between the hours of 9 a.m. and 3 p.m.

OR

Arrange their pickup with our lab staff contact.  See staff list.

Turn-around time

Projects will be started in the order they are received.

The amount of time estimated to complete the genome engineering process will be determined on a case-by-case basis.

Pick-up of produced products

Completed cell lines can be picked up as either a frozen stock or as a live culture provided in a sealed T25 flask from 6-108 MCB.

Cytogenomics

Investigators are encouraged to submit their chosen cell line to the Cytogenomics Shared Resource for G-band Karyotyping to determine the ploidy status and genomic integrity before proceeding with genetic modification by the GESR. 

Location

Location

We are located at:

Laboratory

6-221 Molecular Cellular Biology Building
420 Washington Ave SE
Minneapolis, MN 55455

Office

6-108 Molecular Cellular Biology Building
420 Washington Ave SE
Minneapolis, MN 55455

mcbb map

Staff

Co-directors

Eric Hendrickson, Ph.D
Office: 6-102 MCB
612-624-5988
hendr064@umn.edu

Branden Moriarity, Ph.D.
Office: 3-128 CCRB
612-625-2226
mori0164@umn.edu

Coordinator

Brian Ruis
Office: 6-108 MCB
612-624-0935
612-625-1484
ruisx001@umn.edu

Support staff

Elissa Carrol
Office: 6-108 MCB
612-624-0935
carro317@umn.edu

Huiyan (Summer) Ma
Office: 6-108 MCB
612-624-0935
maxx0326@umn.edu