Research Program: Transplant Biology and Therapy
Associate Professor, Department of Medicine
Staff Physician, Hematology-Oncology, Minneapolis Veterans Affairs Medical Center
Director, Research Flow Cytometry Core Facility, Minneapolis Veterans Affairs Medical Center

gupta013@umn.edu
612-467-4135 — office

Dr. Gupta is an associate professor in the Department of Medicine. He is also a staff physician in the Hematology-Oncology Section and the director of the Research Flow Cytometry Core Facility at the Minneapolis Veterans Affairs Medical Center. Dr. Gupta received his M.B.B.S. (medical graduation) in 1984, M.D. in internal medicine (equivalent to board certification in internal medicine) in 1988, and oncology subspecialty training from the All India Institute of Medical Sciences, New Delhi, India. He received training in bone marrow transplantation at the Royal Marsden Hospital, London, U.K., and subspecialty training in hematology, oncology and transplantation at the University of Minnesota.

Research Interests

Dr. Gupta's clinical interest is in the treatment of myelodysplastic syndromes and gastro-intestinal malignancies. His research interests include (i) the biology of human hematopoietic stem cells, particularly the role of heparan sulfate proteoglycans in the long term ex vivo maintenance and expansion of hematopoietic stem cells, (ii) the mechanisms by which abnormal proteoglycans cause the clinical abnormalities seen in the mucopolysaccharidoses (including Hurler syndrome), and the potential application of multipotent adult cells (MASC) for correction of these abnormalities, and (iii) the pathophysiological mechanisms causing the preleukemic myelodysplastic syndromes (MDS), including apoptosis, marrow stromal function and stem cell-stromal interactions.

Selected Publications

Gupta P, McCarthy JB, Verfaillie CM. Stromal fibroblast heparan sulfate is required for cytokine-mediated ex vivo maintenance of human long-term culture-initiating cells. Blood 1996; 87: 3229-36.

Gupta P, Oegema TR, Brazil JJ, Dudek AZ, Slungaard A, Verfaillie CM. Structurally specific heparan sulfate support primitive human hematopoiesis by formation of a multimolecular stem cell niche. Blood 1998; 92: 4641-51.

Gupta P, Blazar BR, Gupta K, Verfaillie CM. Human CD34+ bone marrow cells regulate stromal production of interleukin-6 and granulocyte colony-stimulating factor and increase the colony-stimulating activity of stroma. Blood 1998; 91: 3724-33.

Gupta P, LeRoy SC, Luikart SD, Bateman A, Morrison VA. Long-term blood product transfusion support for patients with myelodysplastic syndromes (MDS): cost analysis and complications. Leukemia Res 1999; 23: 953-59.

Gupta P, Niehans GA, LeRoy SC, Gupta K, Morrison VA, Schultz C, Knapp DJ, Kratzke RA. Fas ligand expression in the bone marrow in myelodysplastic syndromes correlates with FAB subtype and anemia, and predicts survival. Leukemia 1999; 13: 44-53.

Punzel M, Gupta P, Roodell M, Mortari F, Verfaillie CM. Factor(s) secreted by AFT024 fetal liver cells following stimulation with human cytokines are important for human LTC-IC growth. Leukemia 1999; 13: 1079-84.

Verfaillie CM, Gupta P, Prosper F, Hurley R, Lundell B, Bhatia R. Stromal extracellular matrix components as growth regulators for human hematopoietic progenitors. Hematology 1999; 4: 321-33.

Gupta K, Gupta P, Solovey A, Hebbel RP. Mechanism of interaction of thrombospondin with human endothelium and inhibition of sickle erythrocyte adhesion to human endothelial cells by heparin. Biochim Biophys Acta 1999; 1453: 63-73.

Gupta P, Oegema TR, Brazil JJ, Dudek AZ, Slungaard A, Verfaillie CM. Human LTC-IC can be maintained for at least 5 weeks in vitro when interleukin-3 and a single chemokine are combined with O-sulfated heparan sulfates: requirement for optimal binding interactions of heparan sulfate with early-acting cytokines and matrix proteins. Blood 2000; 95: 147-55.

Gupta K, Gupta P, Ramakrishnan S, Hebbel RP. Binding and displacement of vascular endothelial growth factor (VEGF) by thrombospondin: effect on human microvascular endothelial cell proliferation and angiogenesis. Angiogenesis 2000; 3: 147-58.

Brazil JJ, Gupta P. Constitutive expression of the Fas receptor and its ligand in adult human bone marrow: a regulatory feedback loop for the homeostatic control of hematopoiesis. Blood Cells Mol Dis 2002; 29 (1): 94-103.

Punzel M, Gupta P, Verfaillie CM. The microenvironment of AFT024 cells maintains primitive human hematopoiesis by counteracting contact mediated inhibition of proliferation. Cell Communication and Adhesion 2002; 9 (3): 149-159.

Luikart SD, Masri M, Wahl D, Hinkel T, Beck JM, Gyetko MR, Gupta P, Oegema TR. Urokinase is required for the formation of mactinin, an a-actinin fragment that promotes monocyte/macrophage maturation. Biochim Biophys Acta 2002; 1591: 99-107.

Stringer SE, Nelson MS, Gupta P. Identification of a MIP-1a binding heparan sulfate oligosaccharide of defined length that supports long-term in vitro maintenance of human LTC-IC. Blood 2003; 101(6): 2243-45.

Luikart SD, Krug HE, Nelson RD, Hinkel T, Majeski P, Gupta P, Mahowald M, Oegema TR. Mactinin: a modulator of the monocyte response to inflammation. Arthritis Res Therapy 2003; 5(5): R310-16.

Pan C, Nelson MS, Reyes M, Koodie L, Brazil JJ, Stephenson EJ, Zhao RC, Peters C, Selleck SB, Stringer SE, Gupta P. Functional abnormalities of heparan sulfate in mucopolysaccharidosis-I are associated with defective biological activity of FGF-2 on human multipotent progenitor cells. Blood 2005; 106 (6): 1956-64.

Gupta P. Impairment of the activity of glycosaminoglycan-binding cytokines by functionally abnormal heparan sulfates: a novel mechanism underlying disease pathophysiology. Scientific World J 2006; 6: 452-56.

Garcia-Rivera MF, Colvin-Wanshura LE, Nelson MS, Nan Z, Khan SA, Rogers TB, Maitra I, Low WC, Gupta P. Characterization of an immunodeficient mucopolysaccharidosis type I (MPS-I) mouse model suitable for preclinical testing of human stem cell and gene therapy. Brain Res Bull 2007; 74(6): 429-38.

Kodali D, Mesa H, Rawal A, Cao Q, Gupta P. Thrombocytosis in myelodysplastic and myelodysplastic/myeloproliferative syndromes. Leuk Lymphoma 2007; 48(12): 2375–80.

Hussein K, Jahagirdar B, Rogers T, Gupta P, Burns L, Larsen K, Weisdorf D. Day 14 bone marrow biopsy in predicting complete remission and survival in acute myeloid leukemia. Am J Hematol 2008 (in press).