On this page:

• Equipment Features
  • Flurochrome Chart
• Fees
• Training
  • Required Safety Training
  • Pre-training
  • Training
• Scheduling
• Sample Restrictions
• Publication Acknowledgement Request

Equipment Features

• Fluorescence-activated cell sorter
• User-run
• 488nm Laser

The image scanning cytometer from Amnis is a blend of flow cytometry and microscopy. Image scanning cytometer takes a picture of each cell as it goes through a flow cell. Each cell is broken down into individual fluorescent wavelength or analyzed as a composite. There is a 488nm laser that emits to 6 parameters that can be observed and saved. Below is a chart of some flurochrome possibilities:

Flurochrome Possibilities

Dark Field	Bright Field	500-560nm	560-595nm	595-660nm	660-735nm
1	2	3	4	5	6
SSC	FSC	FITC	PE	7AAD	PE Cy5
		Alexa 488	Cy3	Alexa610-PE	PE Cy5.5
		GFP	DSRed	Acridine Orange	PerCP
		Acridine Orange	Propidium Iodide	Propidium Iodide	PerCP Cy5.5
		YFP		ECD or PE-TX Red	DRAQ 5
		RHO 123			Alexa647-PE
		CFSE			HC RED
		PKH2			Alexa 680PE

Back to top

Fees

• There is a $150 fee for training. If you do not show up for class, you will be charged a no-show fee.

Required Safety Training

Users must provide documentation of Hazardous Chemical Waste Training to the lab safety officer at CCRB 754.

Back to top

Pre-training

These pre-training tasks are required before your training session. If you do not complete the pre-training material in advance, you will be not be able to complete the training, and you will still be charged the training class fee.

• Complete the User Information Form (PDF)
• Read the following and bring these documents to training:
  • Handbook (PDF)
  • Definitions (PDF)
  • Experimental Design and Sample Preparation (PDF)
  • Experimental Template (PDF)
• Read the following (you do not need to print):
  • IDEAS User Manual (large PDF file, doesn't need to be printed)
  • INSPIRE Users Manual_V1_0_X (large PDF file, doesn't need to be printed)

Back to top

Training

• Pre-training and training on the Image Scanning Cytometer are required before users can operate these instruments independently.
• Image Scanning Cytometer training is held on Tuesday and Wednesday, 9 a.m.-12 p.m., each day. The class is held at least once a month.
• Please fill out the Training Request Form to schedule a training session.
• Training fees: There is a $150 fee for training. If you do not show up for class, you will be charged a no-show fee.
• If you do not complete the pre-training material in advance, you will be not be able to take the class and you will be charged for the training.

Back to top

Scheduling

Once trained you can schedule yourself for the Image Scanning Cytometer using the online scheduling form.

Sample Restrictions

Cell populations to be analyzed cannot contain radionuclides or infectious agents. Specifically, it is not permissible to bring cells into the Flow Core for analysis that contain radioactive substances (eg, P-32) or are known to be infected with bacteria, viruses, or fungi. Examples would include cells known to be producing infectious virus such as EBV or HIV. This policy is enforced because flow cytometers generate aerosols, which we cannot contain during analysis or sorting.

Back to top

Publication Acknowledgment Request

The Flow Core receives grant support from the National Cancer Institute, which should be acknowledged in any publications. The following statement is suggested: "We would like to acknowledge the assistance of the Flow Cytometry Core Facility of the University of Minnesota Cancer Center, a comprehensive cancer center designated by the National Cancer Institute, supported in part by P30 CA77598."

• We would appreciate a reprint of your publications.
• We appreciate your acknowledgment of the Flow Core services in your publications.

Back to top