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FV500 Confocal Microscopy Facility
Dyes and Spectrum Table

What Laser to Use:


Table that lists the dyes and corresponding lazers to use.
Actual Spectra for Dyes and Lasers
DYE [excitation/emission] Laser
Ca++ Crimson [590/615] Red HeNe
Ca++ Green-1 [506/531] Argon
Ca++ Green-2 [503/536] Argon
Ca++ Green-5N [506/532] Gr HeNe
Ca++ Orange [549/576] Gr HeNe
Ca++ Orange-5N [5??/580] Gr HeNe
Cy 3 [550/565] Gr HeNe
Cy 3.5 [581/596] Gr HeNe
Cy 5 [650/670] Red HeNe
Cy 5.5 [675/695] Red HeNe
Dil [549/565] Gr HeNe
DiO [484/501] Argon
eGFP [488/509] Argon
FITC [494/518] Argon
Fluo-3 [506/526] Argon
Fura Red [472/657] Red HeNe
MitoTracker [490/516] Argon
PI [536/617] Gr HeNe
Rhodamine-Phalliodin [542/565] Gr HeNe
SNARF-1 (pH ?) [488/530] Red HeNe
Texas Red [595/615] Red HeNe
TRITC [496/520] Gr HeNe
YFP [514/527] Gr HeNe
NOT on list: match closest spectra
AlexaFluor-1 [632/647] Red HeNe
APC [650/660] Red HeNe
APC/Cy7 [650;755/767] Red HeNe
Calcein [494/517] Argon
DS Red [558/583] Red HeNe
YoYo-1 [491/509] Argon