Peter Villalta, Ph.D.
Coordinator
Masonic Cancer Center of the University of Minnesota
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Analytical Biochemistry
Mass Spectrometry Services
The Analytical Biochemistry shared resource provides a variety of molecular identification and quantitation services. Ion trap and quadrupole mass spectrometers are available and can be used in conjunction with liquid (electrospray and APCI ion sources) and gas (EI and CI ion sources) chromatography. The facility typically provides mass spectral data for small molecule identification and quantitation but can also analyze oligonucleotides and whole and digested protein samples. A variety of quantitative assays of samples derived from complex in vitro and in vivo systems are routinely performed. The facility can either work with the user to develop an assay or use an existing assay to analyze the user's samples. Analyses of low level samples can be performed with the enhanced sensitivity of the nanoflow electrospray capabilities of the mass spectrometry facility.
Instrumentation
- 3 Triple Quadrupole LC-MS/MS Instruments
- Thermo Electron Quantum Ultra AM
- Thermo Electron Quantum Discovery Max (2)
- 2 Ion Trap LC-MS/MS Instruments
- Thermo Finnigan LCQ Deca
- Agilent MSD SL
- Single Quadrupole GC-MS (Hewlett-Packard 5973 MSD)
- Triple Quadrupole GC-MS/MS (ThermoFinnigan TSQ 7000
Typical Mass Spectrometry Data
Full scan and fragmentation spectra of a DNA adduct and the LC-MS/MS chromatogram using the selected reaction monitoring (SRM) transition determined from the mass spectra.
Instrument Schedules
- LC-MS
- GC-MS
The billing rate for Cancer Center members is $20/hour for all instrumentation (except for the HP GC/MS instrument which is $8/hour). The minimum billable time is 0.25 hours and times are rounded up to the nearest 0.25 hour. Outside users should inquire about rates applicable to them.
To request mass spectrometry services, please contact Peter Villalta (626-8165, villa001@umn.edu) and fill out the Analysis Submission Form (PDF)
Contact Information
Coordinator:
Peter Villalta, Ph.D.
612-626-8165
villa001@umn.edu
Fax: 612-626-5135
Lab: 795 CCRB