Masonic Cancer Center

A comprehensive cancer center designated by the National Cancer Institute

Analytical Biochemistry

The Analytical Biochemistry shared resource provides state-of-the-art chemistry services and expertise in mass spectrometry, chromatography, and analytical biochemistry. These three complementary specialties afford a center of expertise for a full range of chemical and biochemical services in support of the peer-reviewed research of Cancer Center investigators.

All the services are located in the Cancer and Cardiovascular Research Building at 2231 6th Street SE, Minneapolis, Minn. 44544.

Video On Services Provided by the Analytical Biochemistry Shared Resource

 

 

StaffAHCMCC2 - Image - 150x220 - Robert Turesky

Director
Robert Turesky, Ph.D.
612-626-0141
rturesky@umn.edu
Fax: 612-624-3869
Office: 2-149 CCRB

Analytical Biochemistry services coordinator
Sharon Murphy, Ph.D.
612-624-7633
murph062@umn.edu
Fax: 612-626-5135
Lab: 2-240 CCRB
Office: 2-127 CCRB

Chromatography services coordinator
Steven Carmella
612-626-5235
carme001@umn.edu
Fax: 612-626-5135
Office: 2-144 CCRB
Lab: 2-210 CCRB

Mass spectrometry services coordinator
Peter Villalta, Ph.D.
612-626-8165
villa001@umn.edu
Fax: 612-626-5135
Office: 2-143 CCRB
Lab: 2-210H CCRB

 

The Analytical Biochemistry shared resource offers sensitive and quantitative methods for determining important compounds in human urine, blood, or DNA, including:

  • Nicotine, cotinine, and their metabolites in human urine, blood, or saliva
  • Tobacco-specific nitrosamine metabolites in human urine or blood
  • Polycyclic aromatic hydrocarbon metabolites in human urine
  • Carcinogen-hemoglobin adducts
  • Carcinogen-DNA adducts
     
Contact Information

Coordinator:
Sharon Murphy, Ph.D.AHCMCC2 - Image - 180x222 - Sharon Murphy
612-624-7633
murph062@umn.edu
Fax: 612-626-5135
Office: 2-127 CCRB
Lab: 2-240 CCRB

The Analytical Biochemistry shared resource provides advice and assistance in the development of state-of-the-art chromatographic separations. The focus is on methods using gas chromatography (GC) and high-performance liquid chromatography (HPLC). The chromatography specialist, Steven Carmella, will consult with Cancer Center investigators about method development or application of existing methods to analytical chemistry problems.

The Analytical Biochemistry shared resource has particular expertise in the chromatographic separation and quantitation of metabolites, adducts, and other compounds in biological matrices. Instruments in the facility are available for preliminary methods development studies. These instruments include Waters gradient solvent delivery systems with UV-Vis, fluorescence, and radioactivity detectors.

The chromatography specialist will also assist in solving instrumentation problems which Cancer Center investigators may encounter. The chromatography services function of the Analytical Biochemistry shared resource is a highly efficient and cost-effective solution to separation problems.

Contact Information

Coordinator:
Steven Carmella AHCMCC2 - Image - 180x222 - Steve Carmella
612-626-5235
carme001@umn.edu
Fax: 612-626-5135
Office: 2-144 CCRB
Labs: 2-120 CCRB

Web Links

The Analytical Biochemistry shared resource provides a variety of molecular identification and quantitation services. Ion trap and quadrupole mass spectrometers are available and can be used in conjunction with liquid (electrospray and APCI ion sources) and gas (EI and CI ion sources) chromatography.

The facility typically provides mass spectral data for small molecule identification and quantitation but can also analyze oligonucleotides and whole and digested protein samples. A variety of quantitative assays of samples derived from complex in vitro and in vivo systems are routinely performed.

The facility can either work with the user to develop an assay or use an existing assay to analyze the user's samples. Analyses of low level samples can be performed with the enhanced sensitivity of the nanoflow electrospray capabilities of the mass spectrometry facility.

InstrumentationAHCMCC2 - Image - 180x270 - Peter Villalta
  • Thermo Scientific Orbitrap Velos LC-MS/MS Instrument
  • 5 Triple Quadrupole LC-MS/MS Instruments
  • Thermo Electron Quantum Ultra AM
  • Thermo Electron Quantum Discovery Max (2)
  • Thermo Scientific TSQ Vantage (2)
  • 2 Ion Trap LC-MS/MS Instruments
  • Thermo LCQ Deca
  • Agilent MSD SL
  • Single Quadrupole GC-MS (Hewlett-Packard 5973 MSD)
  • Triple Quadrupole GC-MS/MS (Thermo Scientific Quantum GC)
     

Typical Mass Spectrometry Data

AHCMCC2 - Image - 360x279 - Mass Spectrometry Figure
Full scan and fragmentation spectra of a DNA adduct and the LC-MS/MS chromatogram using the selected reaction monitoring (SRM) transition determined from the mass spectra.

Instrument Schedules

LC-MS

GC-MS

Rates for Use

The billing rate for cancer center members is $20/hour for all instrumentation (except for the HP GC/MS instrument which is $8/hour). The minimum billable time is 0.25 hours and times are rounded up to the nearest 0.25 hour. Outside users should inquire about rates applicable to them.

Contact Information

To request mass spectrometry services, please contact Peter Villalta and fill out the Analysis Submission Form (PDF)

Coordinator:
Peter Villalta, Ph.D.
612-626-8165
villa001@umn.edu
Fax: 612-626-5135
Office: 2-143 CCRB
Lab: 2-210H CCRB

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  • Last modified on December 20, 2013